How can saponins and isoflavones be separated from ethanol extracts?

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Djaafar Zemali

I believe that chromatographic separation techniques such as column chromatography, high-performance liquid chromatography (HPLC), or preparatory thin-layer chromatography (TLC) can separate saponins and isoflavones from each other based on their chemical properties and polarity.

Haile Tesfaye Duguma

Djaafar Zemali Thank you so much for your response. Can the mentioned methods help to obtain saponins and isoflavones in large quantities (grams) to be used for research purposes?

I believe that chromatographic separation can provide relatively high yields of isoflavone saponins from ethanol extracts, especially when optimized methods are used.