I am trying to establish a primary myoblast culture from zebrafish but my cells does not attach after 1st passage. Do you have any suggestions?

Hello eveyone, I am trying to establish a primary myoblast culture from zebrafish but my cells does not attach after 1st passage. I am trying to establish tissue explant culture since I do not...

01 March 2018 7,428 2 View

Why do i observe BFP and GFP on same reporter cell?

I am using 293T-BFP reporter cell. I am doing gene editing with Cas9. 293T-BFP cells have repair template plasmid and when cas9 cuts BFP site on template, BFP converts GFP by adding donor...

15 September 2017 5,546 2 View

What is the relationship between sleeping on the right or left side and health?

Our family and some experts told us that sleeping on the left side is not good for health. However, when I looked on the internet, I read that on the contrary, it is necessary to lie on the left...

01 January 1970 1,218 3 View

Doesn't artificial sun increase drought?

01 January 1970 8,226 4 View

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23 February 2021 4,014 3 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

How to determinate phosphatidylcholine content in cow milk?

I would like to determinate the phosphatidylcholine content in cow milk. We only have a Shimadzu Prominence HPLC (High Performance Liquid Chromatography) with a fluorescent detector for the...

18 January 2021 8,370 6 View

The chemical and microbiological properties of camel milk do have a significant effect on shelf life of camel milk powder compared to cow milk powder?

I need to know effect of heat treatments that are made when making camel milk powder on chemical , microbiological properties and shelf life of camel milk powder compared to cow milk powder. Thanks

11 January 2021 7,097 12 View

Can I use saponin to permeabilize cell to measure mitochondrial respiration?

Saponin and digitonin both can permeabilize cells. However, based on many respiration assay protocols I read, saponin is used for tissue permeabilization and digitonin is used for cell...

11 January 2021 2,151 3 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

How should I store the milk sample in RNAlater?

I want to extract RNA from human breast milk. How should I store the milk sample in RNAlater? And after RNA extraction how can I store the RNA?

01 December 2020 7,183 4 View

Which buffer should I use to aliquot antibody in?

for western blot analysis the buffer in which the antibody already is, written in the datasheet, or should I use already the bsa or milk solution that I use for final diluition?

17 November 2020 4,804 3 View

What is the chemical composition of Cucumis melo roots?

I have found an old pharmaceutical research from 1833 in which emetic power of Cucumis melo roots was confirmed. I cannot find data about chemical constituents of the Cucumis melo roots. Their...

30 October 2020 3,633 3 View

What is the suitable temperature to store Vit A, E, D sample extract extracted from milk powder?

I run the sample preparation using AOAC Vit A,E,D method, that require saponification, concentration , drying using nitrogen gas, reconstitute with methanol. Since the HPLC method development is...

23 October 2020 6,430 4 View