I am carrying out a project of molecular identification of strains that are possibly actinomycetes. However, I am not sure if the primers I should use are for eubacteria or actinobacteria, since they could be another type of bacteria.
You can use universal bacterial primers, such as 27F/1492R to amplify the whole 16S gene (these are widely used, see for example https://www.nature.com/articles/s41467-019-13036-1 ).
If you're using amplicon sequencing, you can use primers that amplify only one or two of the V regions. See for example Article Conservative Fragments in Bacterial 16S rRNA Genes and Prime...
Article Antimicrobial activities of actinomycetes isolated from unex...
You can have a look at this research article and use AB1 and 1378R primer pairs.
What do you think eubacteria or actinobacteria are? Are they different or can they be compared? What are their taxonomic rank?
I recommend you to please look for the taxonomic informations before asking such question.
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