The antibody is already purified from hybridoma and would be kept at 4°c in 2% FBS/0.1 Saponin/PBS, plus the sodium azide (0.02%).
The use for this Ab is for IFA (Intracellular staining), and it is a primary mIgG1.
Thanks a lot!!! :)
Hi there,
0.02% NaN3 is often used in dilutions of primary antibody for Western Blotting. If your staining is performed on living cells NaN3 may have side effect depending on the final concentration. See the following:
http://www.abcam.com/protocols/antibody-storage-guide
Pl. clarify what you actually means "to avoid contaminations in reused Abs". For diluting mAB you can very well use NaN3 concentration that you have mentioned.Keep in mind that you are not performing Intracellular staining in living cells. If you are doing so you have to remove NaN3 prior to its use in Intracellular staining. For staining if you are utilizing peroxidase enzyme you have to throughly wash out NaN3 as it is a inhibitor of peroxidase enzyme activity.
If you reqire further clarification pl. get back to me.
With best wishes
you can also double the sodium azide concentration (0.04%) with no problems. As pointed by Shrivastav you must only wash (carefully three times is enough) in case of further incubation with peroxidase conjugated secondary antibodies.
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