Hector Moreno

4 Questions 3 Answers 0 Followers

Questions related from Hector Moreno

Are samples resuspended in RLT buffer suitable as starting material for TRIzol extraction?

I collected my samples in RLT and stored at -80c. Later on, i am considering to use TRIzol extraction instead of Qiagen's protocol. The question is: may I add TRIzol to the sample and perform the...

12 February 2019 2,853 2 View

Some groups reported IFN induction upon lipofectamine transfection. Does someone know about JetPrime transfection impact in innate immune response?

Some groups reported IFN induction due to lipofectamine-based transfection. In Consequence, some kept using Calcium Phosphate transfection protocol, which seems to affect in a lesser way the...

15 February 2018 9,512 1 View

To avoid contaminations in reused Abs, I am considering to use sodium azide. Has someone put sodium azide in the already diluted Ab?

The antibody is already purified from hybridoma and would be kept at 4°c in 2% FBS/0.1 Saponin/PBS, plus the sodium azide (0.02%). The use for this Ab is for IFA (Intracellular staining), and it...

08 June 2016 7,978 3 View

Proportion of sucrose and supernatant?

Hi everybody! I am doing virus purification through 30% sucrose cushion. My samples are infected cell supernatants. I will use 13ml ultraclear tubes. Does someone knows what could be a good...

25 February 2016 2,183 3 View