Can anybody please suggest a simple method for quantification of Ochratoxin-A from this broth culture?
Ochratoxin A was detected thin-layer chromatography (TLC) on 0.3 mm layers of Adsorbosil 5 silica gel, using toluene:ethyl acetate-90% formic acid (6: 3: 1 vIvIv) for development.
gudluck
:)
Following paper will help you out.
We use next method. OTA was detected by HPTLC (see attachment)
you need a better cleanup for TLC method. Use this one.
I send you further informations for OTA TLC determination in broth culture.
AOAC Official Methods of Analysis, 991.44 (1992)
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