Can someone please suggest the most suitable broth medium for the production of Ochratoxin A by Aspergillus ochraceus NRRL 5175?

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Jéssica Gil-Serna

First of all, check the identification of this strain because I think it has been now classified as Aspergillus westerdijkiae. When we check OTA production in this species we use CYB medium (Czapek Yeast Broth + 5 g/l yeast extract). It usually works well.

Akarapu Rajender Reddy

Information of species.

Aspergillus westerdijkiae Frisvad & Samson

NRRL 5175

Synonyms: Aspergillus ochraceus

Accession numbers in other collections: NRRL A-17098

Isolated from (substrate): sed, cotton seed

Substrate location: College Station, Texas, USA

Identified from: ITS and lsu rDNA sequence

Applications/Products: Ochratoxin A 562 PPM on YES; penicillic acid 3.2 PPT on YES

Comments: Canad J Microbiol 18:631-636. 1972. Good Ochratoxin A producer.