Hi Everyone,

I am a master's student working in a genetic lab using yeast as a model organism. I have been performing a lot of spotting growth assay with different yeast strains to test their growth phenotypes.

One thing that frustrate me was that there were a lot of variability in the growth phenotypes I observed for the same strain in different experiment under the same conditions.

I was wondering if there's any suggestions or tips that I can use to improve the consistency of the result for these growth assays. One thing that I thought about is to keep the experimental condition as similar to each other as possible: i.e. use the ONs that are set up from single colonies of similar sizes, allow the ONs to grow for the same amount of times, Use drug plate that are similar in freshness.

I would love to hear your thought on this. Please let me know if there's any additional information that you might need or if some part of the questions doesn't make sense.

Thank you in advance!