Qihao Liu

5 Questions 9 Answers 0 Followers

Questions related from Qihao Liu

AT-rich site directed mutagenesis troubleshooting help?

Hi everyone, I performing a site directed mutagenesis on a wildtype plasmid and I have been having troubles generating the mutant plasmid I want. I am hopping to make a post here and see if...

22 February 2023 1,327 5 View

[Yeast biology] Alternative method for testing drug sensitivity?

Hi everyone, my model organism is budding yeast, and I am currently looking into testing the effects of some histone H2B mutants in the gcn5Δ background. The main method I am using is serial...

20 September 2021 7,722 1 View

How to optimize alpha factor arrest experiment for budding yeast?

Hi everyone, I am hoping to take a look at the cell cycle progression for my budding yeast and I have chosen alpha factor as the method of cell cycle synchronization. I wish to ask for your help...

07 September 2021 3,730 2 View

Tips for Resolving Phenotypic Variability Observed in Spotting Assay for Growth?

Hi Everyone, I am a master's student working in a genetic lab using yeast as a model organism. I have been performing a lot of spotting growth assay with different yeast strains to test their...

27 October 2020 2,917 0 View

Good Trouble Shooting Tips for Double Digestion?

Hi Everyone, I am a perspective mater's student at UCSD. I been working on cloning and sub-cloning recently, I have ran into some issues; therefore, I just wish to see if there's any good trouble...

31 August 2020 585 10 View