20 September 2021 1 8K Report

Hi everyone, my model organism is budding yeast, and I am currently looking into testing the effects of some histone H2B mutants in the gcn5Δ background. The main method I am using is serial dilution assay (as shown here: https://www.researchgate.net/publication/340837474/figure/fig5/AS:883111411843083@1587561576440/Spot-assay-of-the-viability-of-yeast-cells-treated-with-10-g-L-PAs-b-50-mg-L-vitamin.ppm)

The issue I am experiencing right now is that since the serial dilution assay is relatively qualitative, when the two mutants have similar growth on a drug plate, I have a lot of trouble deciding if there's an effect or it's just caused by random growth. Therefore, I was hopping to get some feedbacks on the alternative methods and experiment I might be able to perform.

Thank you in advance!

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