Mammalian rRNA quantification by RT-PCR?

Hey Guys, Does anyone know where I can find the primer sets for quantification of mammalian rRNA in murine lymphocytes. I looked around and found couple of them in some papers but not all. Does...

06 July 2018 3,309 0 View

To increase the transfection efficiency of naive CD8 T cells with Lonza Nucleofactor Kit?

I am working with Naive CD8 T cells and used LONZA Nucleofactor Transfection kit. The transfection efficiency with plasmids is quite less (30%). Does anyone have better experience with this or...

11 December 2017 8,766 3 View

How to type IFNAR-/- Mouse?

Does anyone know any successful method either PCR or FACS to type IFNAR-/- KO MOUSE?

05 June 2014 1,666 4 View

What statistical equation do I need to put in Standards results from BCA KIT (Thermo) to analyze my result?

The standards are not in a straight line and following a curve so how can I analyze the results accurately? Also I am using GRAPHPAD for my analysis.

04 May 2013 4,585 0 View

Purification of recombinant protein cloned in pET28c using buffer 7.0 by Ni-NTA Agarose.

I have a cell-free extract in K phosphate buffer pH 7.0 and I want to run Ni-NTA column to purify my protein. The protocol says to use buffers of pH 8.0. Is it necessary? Can we try a different...

08 September 2012 5,744 21 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

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22 February 2021 9,932 11 View

What software is capable of opening a file with the extension ".cad" ?

It seems like quick cad was the software but had been discontinued. I tried with other Autodesk software such as fusion 360, AutoCAD. I also tried with QCAD. Please let me know if you know of any...

22 February 2021 9,634 3 View