Piyush Sharma

6 Questions 9 Answers 0 Followers

Questions related from Piyush Sharma

Mammalian rRNA quantification by RT-PCR?

Hey Guys, Does anyone know where I can find the primer sets for quantification of mammalian rRNA in murine lymphocytes. I looked around and found couple of them in some papers but not all. Does...

07 July 2018 3,335 0 View

To increase the transfection efficiency of naive CD8 T cells with Lonza Nucleofactor Kit?

I am working with Naive CD8 T cells and used LONZA Nucleofactor Transfection kit. The transfection efficiency with plasmids is quite less (30%). Does anyone have better experience with this or...

12 December 2017 8,796 3 View

How to type IFNAR-/- Mouse?

Does anyone know any successful method either PCR or FACS to type IFNAR-/- KO MOUSE?

06 June 2014 1,696 4 View

What statistical equation do I need to put in Standards results from BCA KIT (Thermo) to analyze my result?

The standards are not in a straight line and following a curve so how can I analyze the results accurately? Also I am using GRAPHPAD for my analysis.

05 May 2013 4,610 0 View

Thrombin activity in different buffers.

Is there any specific buffer for thrombin activity? I am currently using Tris-cl, pH 8.0 as cleavage buffer, but wish to use Na-phosphate buffer. Does this hamper the activity of thrombin?

02 February 2013 3,526 2 View

Purification of recombinant protein cloned in pET28c using buffer 7.0 by Ni-NTA Agarose.

I have a cell-free extract in K phosphate buffer pH 7.0 and I want to run Ni-NTA column to purify my protein. The protocol says to use buffers of pH 8.0. Is it necessary? Can we try a different...

09 September 2012 5,770 21 View