Mammalian rRNA quantification by RT-PCR?

Hey Guys, Does anyone know where I can find the primer sets for quantification of mammalian rRNA in murine lymphocytes. I looked around and found couple of them in some papers but not all. Does...

06 July 2018 3,309 0 View

How to type IFNAR-/- Mouse?

Does anyone know any successful method either PCR or FACS to type IFNAR-/- KO MOUSE?

05 June 2014 1,666 4 View

What statistical equation do I need to put in Standards results from BCA KIT (Thermo) to analyze my result?

The standards are not in a straight line and following a curve so how can I analyze the results accurately? Also I am using GRAPHPAD for my analysis.

04 May 2013 4,585 0 View

Thrombin activity in different buffers.

Is there any specific buffer for thrombin activity? I am currently using Tris-cl, pH 8.0 as cleavage buffer, but wish to use Na-phosphate buffer. Does this hamper the activity of thrombin?

01 February 2013 3,495 2 View

Purification of recombinant protein cloned in pET28c using buffer 7.0 by Ni-NTA Agarose.

I have a cell-free extract in K phosphate buffer pH 7.0 and I want to run Ni-NTA column to purify my protein. The protocol says to use buffers of pH 8.0. Is it necessary? Can we try a different...

08 September 2012 5,744 21 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View