THLE-2 Media Alternatives?

24 August 2023 0 2K Report

Hi All,

Has anyone had any success culturing THLE-2 cells in media other than the suggested BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit. I have found a couple of references where DMEM with 10% FBS and antibiotics have been used and was wondering if anyone had success using this alternative method?

Thanks

Mayoral R, Valverde ÁM, Llorente Izquierdo C, González-Rodríguez Á, Boscá L, Martín-Sanz P. Impairment of transforming growth factor beta signaling in caveolin-1-deficient hepatocytes: role in liver regeneration. J Biol Chem. 2010 Feb 5;285(6):3633-3642. doi: 10.1074/jbc.M109.072900. Epub 2009 Dec 5. PMID: 19966340; PMCID: PMC2823504.

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