I am performing real-time PCR using cDNA (50 ng), primers (0.2 µM), and SYBR Green Master Mix. However, the reaction and cycle curve I obtained are as shown below. What could be the reason for this, and is there a possible solution?
Look at the scale on the Y-axis. You didn't get any amplification from this sample, you're just seeing baseline "noise".
What does your positive control look like?
just background, take a positive control along with your sample and you will see the difference between a real signal and background.
Also make sure you get only a single band amplified, check it on a gel after 30 cycles. You may not see anything here as the above answers indicate.
I recommend that you run the dissociation curve of the products to corroborate the specificity of the reaction. If you don't have one, you can do that, analyze by electrophoresis. In addition to including the positive control that was recommended to you previously.
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