Dear Colleagues,
I am investigating methods to determine the photodynamic activity of photosensitizers for photodynamic therapy. One of the methods being used is absorption spectrometry. A work concludes that significant absorption of light was shown to be prerequisite but not sufficient for high photodynamic activity. My point of view is: When a photosensitizer absorbs more radiation at a certain wavelength, it will produce more Ros (Reactive oxygen species), i.e the absorption maximum will correspond to the wavelength active photodynamic effect best. However, this point of view contradicts the viewpoint in above work. I look forward colleagues to explain this question.
Thanks in advance.
It looks that your point of view is reasonable, but, probably except this optical reason exist additional mechanism, I don't know, chemical, biological, etc. which play also important role in discussed processes and also influenced on observed results.
Measurements of absorption maxima related to structural flaws in carbon and oxygen groups of chemical structure show decisive part in the exceptional photodynamic activity.
Nguyen, It seems reasonable that the greater the absorption efficiency the greater the release of ROS. This applies to both exogenous photosensitizers and endogenous porphyrins. We are preparing a paper describing the absorption spectra of intact, live planktonic pathogens, both bacteria and fungi, collected with diffuse reflection spectroscopy. (Please see our papers: "The Black Bug Myth" and "Selective Photoantisepsis" posted on RG). I propose that these absorption spectra, if obtained in vivo, will mirror the action spectrum (clinical efficacy VS WL) of the clinical application.
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