Could you get me this report, please?

03 April 2018 7,186 1 View

Hi Please as you know that neutrophils are the only cells that can express Ly6G. So can I ask which antibody is specific for this marker?

actually, some references mentioned to use Gr-1, however, I think it would be inappropriate to use Gr-1 antibody as it was reported before that it contains antibody for both Ly6C and Ly6G whereas...

02 March 2018 8,397 1 View

Why assigning the shell section by selecting middle surface, top surface, or bottom surface to represent the reference surface makes difference?

I'm modeling thin-walled columns using shell element in ABAQUS. I'm using different ways to shape the geometry to end up with the same final configuration whatever the way is. I noticed that when...

09 October 2017 1,061 0 View

Do I need to do Symmetry for beam element?

Dear all, I'm trying to reduce the computational time for a model which contains circular shell element and beam element (with pipe profile) in the top ( see the attached pics). Do I need to do...

05 June 2017 8,630 3 View

What are the parameters should I input for this UMAT code to get results similar to ABAQUS GUI?

I just wrote the Isotropic Hardening rule Fortran code (attached below), which is similar to what's available in ABAQUS library, if you have experienced with this code before, what are the...

31 December 2016 5,980 3 View

What's the procedure to get the original written data from a figure in published paper?

I'm wondering if there is a way to get the original data for some figures in published research papers? I've tried to contact the authors, but no responses come back, could I get this data from...

11 December 2016 9,775 2 View

How can I export or import part, sketches or assemblies from ABAQUS?

I want to use the same geometry that I drew it in ABAQUS and import again in a different ABAQUS model (not copying). I'm trying with File-export to save it, but when I'm gonna import it again, it...

09 October 2016 6,450 4 View

Link ABAQUS 6.14 with intel parallel studio XE 2016?

09 October 2016 2,061 5 View

Thin-walled column under constant axial and lateral cyclic loading?

09 October 2016 5,724 7 View

How can I model thin-walled steel column under constant axial and alternating horizontal load under displacement control at the top of the column?

Hi all, I'm trying to model this geometry (Fig. 5 in the attached file) using kinematic hardening in ABAQUS, and I have been stopped for unknown reason, Could you please guide me especially how to...

08 September 2016 5,937 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View