Could you get me this report, please?

03 April 2018 7,186 1 View

The most common types of bacterial contamination that affecting the growth of anadara granosa or Blood Cockles?

Dear all Professors, Researchers, and Colleagues, I need your help by list ''What are the frequent types of bacterial contamination that affecting the growth of anadara granosa or Blood Cockles...

10 November 2017 1,943 0 View

Why assigning the shell section by selecting middle surface, top surface, or bottom surface to represent the reference surface makes difference?

I'm modeling thin-walled columns using shell element in ABAQUS. I'm using different ways to shape the geometry to end up with the same final configuration whatever the way is. I noticed that when...

09 October 2017 1,061 0 View

Do I need to do Symmetry for beam element?

Dear all, I'm trying to reduce the computational time for a model which contains circular shell element and beam element (with pipe profile) in the top ( see the attached pics). Do I need to do...

05 June 2017 8,630 3 View

What are the parameters should I input for this UMAT code to get results similar to ABAQUS GUI?

I just wrote the Isotropic Hardening rule Fortran code (attached below), which is similar to what's available in ABAQUS library, if you have experienced with this code before, what are the...

31 December 2016 5,980 3 View

What's the procedure to get the original written data from a figure in published paper?

I'm wondering if there is a way to get the original data for some figures in published research papers? I've tried to contact the authors, but no responses come back, could I get this data from...

11 December 2016 9,775 2 View

How can I export or import part, sketches or assemblies from ABAQUS?

I want to use the same geometry that I drew it in ABAQUS and import again in a different ABAQUS model (not copying). I'm trying with File-export to save it, but when I'm gonna import it again, it...

09 October 2016 6,450 4 View

Link ABAQUS 6.14 with intel parallel studio XE 2016?

09 October 2016 2,061 5 View

Thin-walled column under constant axial and lateral cyclic loading?

09 October 2016 5,724 7 View

How can I model thin-walled steel column under constant axial and alternating horizontal load under displacement control at the top of the column?

Hi all, I'm trying to model this geometry (Fig. 5 in the attached file) using kinematic hardening in ABAQUS, and I have been stopped for unknown reason, Could you please guide me especially how to...

08 September 2016 5,937 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View