The minimum change of amino acids and glutamine on culture media (DMEM) of diferent companies, can generate substantial change/adverse effect/alterate macrophages RAW 264.7?
Because every company have an specific formula...
Joshua K. Hughey
If you're asking if a minimal change in concentration of media components can affect macrophage stasis, the answer is yes. I've even found that the macs I work with seem to do better with certain brands of media components, particularly FBS. I'd start with the formula recommended by the company, then just experiment with component concentrations and/or various brands.
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