Does anyone try to use the Q-PCR method to check the titer of retrovirus (MSCV) using the kit? especially the Q-PCR method. I can't find any product that is suitable for this. Does anyone do this before and got some kits suggestion?
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
05 August 2024 8,836 2 View
In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?
05 August 2024 10,034 1 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...
03 August 2024 6,070 3 View
HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...
31 July 2024 5,309 7 View
I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...
31 July 2024 9,697 0 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Please suggest basic literature on lattice-based cryptography. A kind response from the cryptographic community will be highly appreciated.
24 July 2024 6,291 4 View
I want to know that n-doped side of solar cell is connected with positive or negative electrode.
20 July 2024 1,348 2 View
Dear colleagues, I tried to transduce mouse osteoblastic cells with retrovirus many times but I failed, do you have any recommendations? Thank you in advance.
25 November 2023 1,643 0 View
Several members of my lab have been attempting to perform a retrovirus or lentivirus spinfection into CD3 T cells (mouse and human). The ideal plate would be a untreated 12-well plate with the...
18 July 2023 7,189 0 View
Hi, We are using Sartoflow Smart(Sartorius) to concentrate the retrovirus(RVs). RVs were produced in 10%FBS/high glucose DMEM and concentrated by using the 300 kDa of Sartocon Hydrosart...
09 July 2023 7,949 0 View
This plasmid is quite old and has been stored at -20c since 2008. When I miniprep it from liquid cultures of about 5ml, it always runs slower on a gel, including the digested fragments. I also...
18 June 2023 8,945 2 View
Hello, I have recently read about the reverse transcription activity of one of the subunits of telomerase. As far as I know, all reverse transcriptases so far are of viral origin, including the...
13 April 2023 5,870 2 View
What is the best culture medium for HSPC culture? and what is the best ratio of retrovirus/cells for the viral induction to HSPC cells? Any suggestion or reference will be much appreciated.
22 March 2023 2,230 1 View
Hello, I am trying to generate a mutant with the following retrovirus plasmid, but my cells are not resistant to G148. Can you please help me with any tips/suggestions? -pLNCX chick src...
17 January 2023 6,754 0 View
Hi all, I am struggling to obtain any iPSc colonies with my routine reprogramming by infection. To give you more background my cells are MEFs p53 KO with a floxed allele for TRF1, an ubicuous Cre...
20 July 2022 5,019 1 View
Which NGS technology (if viable) would be most useful in confirming presence/absence of virus contamination in cell banks used for drug production? I imagine it would include WGS of host cell...
05 July 2022 9,016 0 View
I'm currently working on a retrovirus. I'm having trouble detecting protein using IP-WB methods. I have the antibody-it's polyclonal, but I don't have antibodies conjugated with beads for the IP...
19 April 2022 625 7 View