How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Can the Dunn's test be used as an appropriate post-hoc for the G-test?

Research Question: Are red grouse (Lagopus lagopus scotica) a statistically significant proportion of raptor (bird of prey) diets? I am planning on using a series of G-tests (determining whether...

23 February 2021 5,827 3 View

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23 February 2021 4,014 3 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

Has anyone had weird polybrene toxicity during viral transduction?

Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...

26 January 2021 4,071 3 View

Does anyone have experience in infecting suspension cells (like THP-1 or K562 etc) by retrovirus?

I am trying to make stably expressing cell line of my gene of interest in Suspension cell line using retrovirus but i don't have recommended protocol. I have searched literature but mostly they...

14 December 2020 8,274 3 View

Hi, what is the best immortalization kit for mouse primary cells?

What about XMRV retrovirus immortalization kit and it is efficiany rate? Do you recommed another kit... Thanks in advance

11 December 2019 2,704 1 View

Will the GP2-293 cells tranfeced with pBABE-GFP and ecotropic pakaging plasmid be green?

I am making retrovirus with pBABE-GFP and ECO packaging vectors in the clonetech packaging cell line GP2-293. I noticed that my host cells are not green. Is this ok?

09 October 2019 3,802 2 View

Is PT67 cell line [10A1 MLV based retrovirus packaging cell line] or pCL-10A1 retrovirus packaging vector available in your laboratory stocks?

Is it available in your laboratory? PT67 cell line = 10A1 MLV based retrovirus packaging cell line or pCL-10A1 vector= retrovirus packaging vector

03 April 2019 9,777 0 View

What are the methods to know the retrovirus positive transduced cells after infecting with retrovirus and antibiotic selection?

Hi I generated retrovirus by transfecting the plasmid with my gene of interest in packaging cell line and checked my gene in generated retrovirus by RT-PCR. When I infecting HEK 293 cell line with...

17 February 2019 9,568 3 View

How to establish AAV-producing stable sf9 cell line with rep and cap gene incorporated into the genome?

I know that there is publicated Bac2.0 system for stable sf9 cell line, but they just use the plasmid transfection and screen the cells with antibiotics. I am wondering whether there is a method...

12 February 2019 2,645 2 View

How to establish AAV-producing stable sf9 cell line with rep and cap gene incorporated into the genome?

I know that there is publicated Bac2.0 system for stable sf9 cell line, but they just use the plasmid transfection and screen the cells with antibiotics. I am wondering whether there is a method...

01 February 2019 2,477 2 View

What are the methods to know the retrovirus positive transduced cells after infecting with retrovirus and antibiotic selection?

Hi I generated retrovirus by transfecting the plasmid with my gene of interest in packaging cell line and checked my gene in generated retrovirus by RT-PCR. When I infecting HEK 293 cell line with...

01 February 2019 2,628 3 View

How do you detect GFP reporter fluorescence after fixation?

11 December 2018 8,948 5 View