Can someone help me with Retrovirus production?

18 January 2023 0 7K Report

Hello,

I am trying to generate a mutant with the following retrovirus plasmid, but my cells are not resistant to G148. Can you please help me with any tips/suggestions?

-pLNCX chick src Y527F

-pUMVC

-pCMV-VSV-G

Thank you so much for your help!

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