Does anyone know the ISO standard that is useful for Bradford assay?
I've designed media for CHO cells, without Glutamine! But when they're in large scale, the cells were damaged and decreased their viability. What's your refer ?
14 January 2024 2,503 2 View
How can I find "PCR markers" and "suitable antibody to Flow cytometry" of C2C12 cell line? Is there any web server to find this data?
02 December 2023 3,248 0 View
Can someone provide me with the earthquake repositioning code with Python?
29 September 2023 6,469 3 View
Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...
22 July 2023 964 6 View
hello, I have a major question that i would really appreciate if anyone could make me clear. In a project I am going to transfect BMSCs with a plasmid by lipofectamine 2000 reagent. then the...
06 June 2023 9,768 1 View
I want to use an epilepsy database ( CHB-MIT Scalp EEG Database) and the samples have some bipolar channels like FP1-F7, F7-T7, T7-P7, P7-O1, FP1-F3, etc. We can define monopolar channels in...
12 July 2022 3,546 0 View
I dont mean bacterial cellulose(the cellulose which produce by bacteria like G.xylinus ) or the cellulose fibril that present in paper mill activated sludge, i mean a protocol for extracting the...
02 July 2022 6,182 2 View
Is there any free available tool or even demo that gets the MRI image and creates the contouring of different organs? I need to just test some few cases. Thanks.
07 March 2022 1,689 0 View
Hello everyone I am working on a human cell-line of Oligodendrocyte Precursor cell (OPCs) called MHB. Results of my immunocytochemistry experiments showed that these cells express NG2, and don’t...
15 December 2021 3,208 1 View
I have been searching the publications reporting STR markers in the genomic neighborhood of my interest, but I wonder if there might be a more straightforward approach to it. Also within the UCSC...
23 November 2021 8,236 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
In 2018 the OHSAS 18001 was improved and progressed into ISO 45001. This was characterized by one or two short falls. I am now reviewing the ISO 45001, with the aim of looking at the entire...
18 July 2024 9,489 0 View