I did the IHC staining on human frozen tonsil using the conditions that were tittered on the cells. The fixation with acetone destroyed the tissue so the IHC staining looks horrible L
I could fix with NBF or methanol/ethanol but the tissue panel and the TMA are both fixed in acetone (that is why we tested this) – any thoughts?
Hi Pri,
Maybe my remark is close to stupidity, if so, I'm sorry :). Do you need to fixate the sections? We do a lot of IF staining on frozen sections. Instead of chemical fixation we do the staining on (air dried) sections. Embedding (after the staining procedures, with either PBS/glycerol or Slowfade.
Why don't you try 10% neutral buffered formalin? I am sure you will be happy to resolve the problem simply, without much ado!
If you can get full text of this article, it would assist immensely. The issue of believing fixing frozen tissue/section with acetone is an evolving shifted paradigm as a gold standard. https://doi.org/10.1080/10520290701375302
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