Dear researchers;
what is the difference between animal vivarium and animal shelter? which one is used for the plane in animal lab that mice were kept?
I imported MR images of some patients into 3D slicer, including T1, T2 images. Now I want to segment tumor in every image. When I do it for one patient, the segmentations overlay on all images of...
10 November 2019 5,755 3 View
I need to extract miRNA from blood plasma in glioma patients. I did it by GeneAll kit but RNA yield is low. Who can help me to improve the yield by using this kit or to find a manual method that...
11 December 2018 5,140 15 View
I want to find the price of some nuclear medicine instruments. Who ca help me to find this information?
02 March 2015 8,850 2 View
I need the density of this ferrite (m/V). Who has calculated it before or has a suggestion?
01 February 2014 5,367 4 View
I need a good thermometer for measuring temperature of nanoparticles and mouse skin in a AC magnetic field. I need the available models with moderate price for temperature range: 25-70 centigrade...
31 December 2013 7,764 7 View
I wanted to work on magnetic fluid hyperthermia (in-vivo study) with an alternative magnetic field but I faced a problem with finding a good coil for it. Now I have my magnetic nanoparticles and I...
31 December 2013 7,447 6 View
You use of "two nonet chiral: M & M' " and their transformation under U(1)A, at the quark level, and also under left handed and right handed unitary transformation: (SU(3)R *...
31 December 2013 5,174 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View