The Diagnosed MSUD patient is on leucine, isoleucine & valine free diet. Can that patient show normal blood level of leucine and valine amino acid?

24 February 2022 0 2K Report

A 3 months diagnosed male child of MSUD is on formula diet which is leucine, isoleucine & valine free and showed normal leucine and valine values on blood LCMS/MS profile

Badges
Science topic

More Ashwini A. Yadav's questions See All

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Which indexation is better Scopus or Web of Science?

Both are very repetitive indexing agencies.

26 February 2021 9,455 4 View

Anybody write me the name of scopus journals in commerce, management, economics areas. Which publish without any charge?

Please write those scopus journal names for which you have good experience with them.

24 February 2021 6,210 1 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

How to decrease the time for publication of paper in a journal ?

It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.

17 February 2021 2,997 14 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View

Do you think NCC and 3 year service at army base must be compulsory to make the students loyal and discipline to the nation?

Decipline and loyalty for nation is the need of today.

09 February 2021 6,928 3 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

What is India's InternationalTrade Policy during Pendamic? How do you find the policy as successful one?

During Covid-19 Pendamic Government are facing problem to maintain business. India is one of them so this question is perfect to be discussed here.

08 February 2021 2,725 3 View

What is the role of IQAC to improve the grading of an institution?

IQAC provides all information about institution and its various activities to NAAC, Bangluru, India in order to get good grading. In this light what is the role of IQAC??

03 February 2021 6,916 1 View

Best method for immunostaining FLAG-tagged LRRK2 in rodent brain?

I have been carrying out IHC to stain FLAG in fresh-frozen rat brain using FLAG M2 (F1804) with ABC then DAB, but am unable to identify positive staining, and seeing a high background. I carried...

08 October 2020 8,986 2 View

Why Leucine is used for bacterial production measurement?

Leucine uptake assay is widely used for bacterial production. But I have three concerns (1) Why Leucine (not other amino acids) is used and (2) In case of environmental samples, Leucine may be...

25 February 2020 7,758 6 View

Can anyone provide me the literature associated with Leucine Amino Peptidase (LAP) Assay, (Adamor etal, 1963) ?

Leucine Amino Peptidase (LAP) Assay protocol

11 December 2019 7,021 3 View

How to elute the proteins completely after immunoprecipitation?

Dear All, I am using Anti-Flag magnetic beads for immunoprecipitation experiments. I want to perform elution under the non-denaturing conditions for further use of proteins as well as to reuse...

10 November 2019 4,806 7 View

How can I store p-dimethylaminocinnamaldehyde solution, l-leucine b-naphthylamide solution and p-naphthylamine stock solution?

Hello, The aforementioned solutions are to be used for the purpose of conducting soil enzyme assays. Because I will be using the solutions frequently in the coming weeks, I plan to store them....

19 February 2019 6,589 7 View

Is Enhanced GFP post-transcriptionally modified?

Dear all, I want to design a microRNA that targets EGFP to downregulate its expression. I find that the actual DNA sequence of EGFP codes for a Valine that would be the second amino acid, but...

10 November 2018 5,638 3 View

Is Enhanced GFP post-transcriptionally modified?

Dear all, I want to design a microRNA that targets EGFP to downregulate its expression. I find that the actual DNA sequence of EGFP codes for a Valine that would be the second amino acid, but...

07 November 2018 7,599 3 View

Leucine 60 to proline mutation in GFPmut3?

We wanted to tag a protein of interest with GFP mut3 version. upon generating the construct and sequencing it we found it has an additional mutation. A conserved leucine at 60th position had got...

07 August 2018 7,631 0 View

Isoleucine and Leucine as equivalent in peptide sequence in uniprot?

May I know is there any significance in treating Isoleucine and Leucine as equivalent in peptide sequence searches? Does it affect the results of the search in finding the 'right' protein if we...

03 April 2018 2,342 0 View

How could alanine substitution vs deletion have an impact on a protein?

I performed deletion mutagenesis along the c terminal region of a protein targeting two specific leucine residues, and showed no change compared to wild type, as assayed by dual...

05 February 2018 4,380 2 View