After condensation of amino acids (they do not contain any protection groups), I believe that I have produced leucine peptides such as di-leucine, tri-leucine, leucine diketopiperazine, tetra-leucine and also unreacted L-leucine. I am using a C18 column, 250x4.6 mm with 3um particle size. Is there an aqueous mobile phase that can ion-pairing to separate oligomers with different length?
Jack Silver
What have you tried already, and what were the results? You might try an AQ type column using TFA as a solvent modifier. I suspect the compounds are very polar and retain poorly on regular C18
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