Hello
I'm trying to do amino acid analysis in my rice germ extract. I want to measure lysine and valine content but the GABA content in this particular extract is very high, hence other peaks are hard to see.
I'm pretty new at HPLC, so I was wondering if anyone have any suggestion on how to measure low content amino acid ?
Thank you
Paul Körner
What do you mean by "other peaks are hard to see"? Do your analytes co-elute? Then adapt your HPLC method so that they seperate sufficiently from each other. If they do not co-elute, perfect, zoom-in.
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Ricarda Koopmann
http://bibd.uni-giessen.de/gdoc/1999/uni/d990021.pdf
I just found this dissertation about food chemistry, perhaps it can be helpful for your question.
GABA should not give a signal after this derivatization reaction.
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