The best protocol for Alzheimer induction in rat?

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How to compare different models with different inputs and data length?

I want to run 5 different models to estimate stream flow. In order to optimize the characteristics of these models I use Taguchi method. So I have to run different models according to the Taguchi...

11 February 2021 7,986 6 View

How to find strain energy release rate GI, GII, GIII in Abaqus?

I modeled a simple crack in abaqus. I get J-integral as output as well as components of stress intensity factor KI, KII, KIII. However, I do not know how to get GI, GII and GIII. I searched in...

04 January 2021 1,783 2 View

What is the best way of moscaicing overlapping images?

I have several images with overlaping areas. I try to mosaic them using rasterio.merge library. But rasterio.merge funtion replaces those overlapping pixels with "nan" values. I then tried both...

29 December 2020 9,849 1 View

How can I check the correlation between a group of features and one feature?

Hello all, I have a dataset that contains some pressure meters in a water distribution network and all valves of the network. Since I do not have the graph of the water distribution network, I...

30 October 2020 2,333 4 View

Any idea why my Western Blot Stripping buffer doesn't work?

I activated my membrane twice in total and hybridized it with two different antibodies. The first antibody was the specific antibody I was looking for and the second one was GAPDH. I then...

29 October 2020 5,530 6 View

Changing TEM mode to TE and TM modes?

Hi there, I would be grateful if someone could let me his opinion about this question: There is a coplanar stripline waveguide excited with a TEM mode. My question is that how to create TE and...

11 October 2020 5,816 3 View

I have problem with my SDS gels in western blot and wondering anyone could help?

So when I prepare my 10% SDS gel for Western blot, I see that my resolving solution is perfect but my stacking solution does not seem to be working properly. So when I take the comb out, the wells...

17 September 2020 3,355 7 View

How can we optimize a water distribution system if we do not have the zones' topology (pipes graph )?

Hello everyone, My issue is about a water distribution system that I am working on a zone of the system where does not exist any plan for the place of pipes. However, we have the place of...

14 September 2020 5,978 6 View

Whats this plant?

Dear All Hi I wanted to know the scientific name of this plant species. would you please help me to identify it. Actually, I found it in the area in the East of Iran.

21 August 2020 6,705 2 View

Which version of Mplus is suitable to assess the exploratory factor analysis of a questionnaire with 20 items?

Dear Researchers and statistical analysts, Which version of Mplus is suitable to assess the exploratory factor analysis (EFA) of a questionnaire with 20 items? I'm working on initial validation of...

14 August 2020 3,341 4 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

Siddhartha Dutta

There are multiple models for inducing AD. As AD is a complex neurological disorder and most of the models do not completely recapitulate the genetic and epigenetic complexity of sporadic AD. Now with newer development of models I guess transgenic mice as AD-models are better

Some of the models are:

Intrahippocampal amyloid infusion model

Intracerebroventricular Ab infusion mode

ferrous sulfate heptahydrate and L-buthionine-(S, R)-sulfoximine

Rat models of cholinergic-dysfunction

Judy Walton's method using aluminum compounds in the water of old rats

Aβ-based models of AD

Transgenic rats

1. The TgAPPswe rat

2. The UKUR25 rat

3. The Tg6590 rat

4. The triple transgenic rat

5. The APP21 and APP31 transgenic rats

6. The AD-tau rat

Nisha Kuzhuppilly Ramakrishnan

Rats do not develop AD and there are no rat models of AD. There are rat models which have particular characteristics of AD that you might be interested in researching. What are you interested in? Tau, Abeta, metabolism, vascular dysfunction, memory loss, cell death....