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Questions related from Nisha Kuzhuppilly Ramakrishnan
Has anyone found any differences in the blood brain barrier permeability of C57Bl/6 and balb/c mice? We will be looking at the brain permeability of a compound after intravenous injection. Is...
10 October 2017 2,864 2 View
I need to evaluate how much of an administered compound is present in mice adipose tissue. Does anyone have a protocol or paper that they can recommend on how to extract a compound from adipose...
04 October 2017 7,133 3 View
We have been performing a thioflavin-T dose-response curve on alpha-synuclein fibrils with very consistent results for some time now. Recently, we received a new batch of fibrils produced by the...
05 October 2016 9,623 1 View
We have only used frozen sections for autoradiograpy with PET tracers so far. One of our collaborators is interested in testing some paraffin embedded sections. Can they be used? Has someone...
13 July 2016 3,625 8 View
My Jaszczak phantom still has a little liquid remaining in it. It only has one opening. I have shaken it to remove most of it and also used a bit of cotton to get most of it out by capillary...
28 January 2016 2,641 2 View
As per literature, the excitation wavelength of alpha-synuclein is around 440nm (and the emission wavelength 485nm). In our setup we have a fixed concentration of the fibrils and thioflavin T...
29 June 2015 5,789 5 View
Which 96-well plate is recommended for alpha synuclein fluorescence in vitro assay with Thioflavin T? Black or white, clear bottomed, low binding, half area etc are some of the descriptions I came...
01 May 2015 6,094 10 View
I am looking for agents that definitely bind alpha synuclein (fibrils), and could potentially be displaced by novel compounds, inorder to set up an in vitro assay for alpha synuclein. So far I...
08 April 2015 8,211 2 View
