Hello,
I have been trying to use the amplex red glucose assay kit (A22189) to measure the glucose in adipose tissue, but when performing it, the standards and all the samples don't show change in the colour, and when analyzing the results, the negative control have an high value of fluorescence. Can someone have an idea what could be happening, or some protocol that I could try?
Thank you
Hello. Do you want to measure the amount of glucose in adipose tissue, simply as one metabolite? Or do you want to evaluate glucose uptake? The answer will differ depending on that.
Thanks for the info. Glucose is metabolized immediately, so you should look at the 2-DG uptake.
This method is easiest and most reliable using RI labeled 2-DG, but colorimetric and chemiluminescence detection is also available.
(However, I only have experience with RI-based method.)
*Colorimetric method
https://www.cosmobiousa.com/products/2-deoxyglucose-2dg-uptake-measurement-kit
*Chemiluminescence
Glucose Uptake-Glo™ Assay
Please carefully examine the methods.
Good luck!
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