TCEP does not work well with Robust Taq - what to do?

More Constance Ekdahl's questions See All

Could this be a case of plagiarism?

I notice questionable similarity between this 2021 conference paper: Johnston, S., & Eamer, L. D. (2021, December). The Paradox Of Being A 50-Year-Old ‘Early Career’Black Female Academic. In...

10 April 2024 663 2 View

I really need your help regarding paper placement. Ideas?

I have an unusual problem in deciding how to place this article I wrote with two colleagues. I am a psychological trauma expert, but also a statistician. During COVID, as my students turned to...

07 March 2024 8,996 3 View

What can someone tell me about Achalasia??

I have a niece, age 40, who has been ill for a few years now and could not get a diagnosis for her condition, until now. She is dying, literally starving to death. She recently (within the last...

30 November 2022 5,178 2 View

Will dried blood be re-suspended when adding buffer/PBS during DNA extraction?

Hi all, I am extracting DNA from blood, and due to waiting for buffer solutions to arrive, the blood samples appear to have dried in their plates. Some were clotted, so have had EDTA added to...

18 March 2019 5,025 3 View

Protocol for protein extraction?

Hi everyone! Does someone have an extraction protocol for protein extraction from muscle tissue? At this moment I am using protease inhibitor, water and Triton. Better ideas? Thank you! Constance

01 January 2019 7,280 7 View

Research topics in renewable energy?

i have a background in psychology,geography and sustainable local development. i want to pursue phd in renewable energy in East Africa from the perpective of social acceptance ,local skills to...

18 April 2018 7,460 4 View

Anyone who can host me in their lab to learn ICP measurement protocol?

I would like to measure intracavernosal pressure in rat penis using the ICP/arterial pressure ratio. I have not done this before and would like to visit a lab that does this routinely - to learn....

25 February 2017 9,408 2 View

Is it relevant to passivate stainless steel under an electric field ?

We are trying to improve an industrial process to treat the surface of several grades of stainless steel (electropolishing + passivation on AISI 304, 316, etc.). I think I read in a study...

03 May 2015 8,386 2 View

At what point does the signal change from electrical to biochemical for transport to the brain?

Is it the retina? So many people are blind because of retinal damage/degradation, but have perfectly healthy optic nerves and brain function. Is there a way of injecting light or some type of...

01 January 1970 7,796 2 View

What statistical test can I use to compare two variables?

One variable is a group of teachers response to a survey, the other will be the principal response. When I run a Pearson r I get a message about the one variable being constant. There is only one...

01 January 1970 5,520 7 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View