Hi,

I would suggest this article that explained very well about the preparation and stability of chitosan nanoparticles.

https://www.sciencedirect.com/science/article/pii/S0378517313006601

Dear all, chitosan nanoparticles are among the most widely investigated substances. A wide bibliography is available. Following some interesting references. My Regards

https://preview.tinyurl.com/y3sjw3zy

https://doi.org/10.1016/j.foodhyd.2018.05.010

https://link.springer.com/article/10.1007/s10311-017-0670-y

https://www.tandfonline.com/doi/full/10.1080/21691401.2018.1533846

https://doi.org/10.1039/C6RA06563E

https://www.nature.com/articles/srep22260

https://doi.org/10.2147/DDDT.S99651

https://doi.org/10.1155/2016/5379424

Chitosan nanoparticles form only after you completely dissolve chitosan in acetic acid which normally require continuous mixing overnight. After that you will need to introduce a polyelectrolyte that is negatively charged like tripolyphosphate. Only then you will induce nanoparticle formation. Here is a good reference that has been cited around 500 times so far.

Article A novel approach to prepare tripolyphosphate/chitosan comple...

Hi, Samiah Mahboob

I suggest you have a look at the following paper that can give a reasonable answer to your interesting question:

Article Chitosan nanoparticles preparation and applications

Best regards, Mahmoud Basyouni

Hi,

https://www.researchgate.net/publication/323775582_Preparation_of_Chitosan_Nanoparticles_and_their_In-vitro_Characterization

https://www.researchgate.net/publication/269718956_A_method_for_top_down_preparation_of_chitosan_nanoparticles_and_nanofibers

Dear Researcher,

You should dissolve chitosan nanoparticles in acetic acid with continuous mixing overnight. Thereafter, you must apply polyelectrolytes such as tripolyphosphate and glutaraldehyde under excellent sonication in order to fabricate globular chitosan nanoparticles.

Dear all, I am so grateful for the precious suggestion. I have been successful in synthesizing the chitosan nanoparticles. I took help from your suggestions and was successful.

Thank you very much.