Best way to dissolve 4-azido-L-Phenylalanine to add it to E.coli culture?

Hello researchers, I am planning to express a protein with non natural AA. When I previously expressed the protein, I found the protein expression to be very low, based on research I found the...

08 September 2016 4,494 5 View

Best way to modify the pH in the protein sample?

We are planning to carry out a protein cleavage reaction, we would like to see if the cleavage reaction occurs in acidic pH (5.5). The protein has been purified, concentrated and saved in pH 8...

07 August 2016 8,234 4 View

Protein conjugation efficiency calculation?

Hello, I am planning to carry out an conjugation reaction between two purified proteins (P1: 59.8KDa and P2: 32.8KDa) to observe the conjugation efficiency on an SDS-PAGE gel. I want to carry out...

07 August 2016 4,133 1 View

Good literature about Antibody conjugations?

Hello everyone, Can anyone suggest me books/scientific articles that discussion about the different conjugation techniques applied in preparation of antibody drug conjugates. Thanks for your...

07 August 2016 7,503 4 View

Methodologies of sequencing a plasmid of no details?

Hello, Any suggestions on how to obtain the sequence of beta-lactamase a & w genes in the plasmids. This plasmids have been donated to our lab and now we want to sequence them to develop an...

01 February 2015 8,291 4 View

Expression of a modified M13K07 phage vector?

We have extracted and modified the M13K07 helper phage genome in gene VIII. Now we have M13K07 modified phage genome (phage vector). Can anyone help me, how to express this phage (as this phage...

07 August 2014 4,230 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View