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Questions related from Nagarjun Kasaraneni
Can anyone provide me an advice on what is the best and easy way to sort the single cell culture from clumpy cells (20 - 40 microns). This cells have been adapted from adherent to suspension...
06 June 2018 8,309 2 View
Hello researchers, I am planning to express a protein with non natural AA. When I previously expressed the protein, I found the protein expression to be very low, based on research I found the...
09 September 2016 4,608 5 View
We are planning to carry out a protein cleavage reaction, we would like to see if the cleavage reaction occurs in acidic pH (5.5). The protein has been purified, concentrated and saved in pH 8...
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Hello, I am planning to carry out an conjugation reaction between two purified proteins (P1: 59.8KDa and P2: 32.8KDa) to observe the conjugation efficiency on an SDS-PAGE gel. I want to carry out...
08 August 2016 4,270 1 View
Hello everyone, Can anyone suggest me books/scientific articles that discussion about the different conjugation techniques applied in preparation of antibody drug conjugates. Thanks for your...
08 August 2016 7,568 4 View
Hello, Any suggestions on how to obtain the sequence of beta-lactamase a & w genes in the plasmids. This plasmids have been donated to our lab and now we want to sequence them to develop an...
02 February 2015 8,356 4 View
We have extracted and modified the M13K07 helper phage genome in gene VIII. Now we have M13K07 modified phage genome (phage vector). Can anyone help me, how to express this phage (as this phage...
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