Dear Mabasa, many thanks for posting this interesting technical question on RG. For more information please have a look at the following potentially useful links which might help you in your analysis:

What is ROX and why use it in qPCR?

https://signagen.com/blog/2019/05/20/what-is-rox-and-why-use-it-in-qpcr/

Also please see:

The Purpose of ROX Reference Dye in Real-Time PCR (qPCR)

https://www.thermofisher.com/blog/behindthebench/the-purpose-of-rox-reference-dye-in-real-time-pcr-qpcr/

I hope it helps. Good luck with your work and best wishes!

Thank you Professor Frank T. Edelmann.

There is 6-ROXtra™ fluorescence reference from AAT, which is better than Rox.

https://www.aatbio.com/products/6-roxtra-fluorescence-reference-solution-25-um-for-pcr-reactions?unit=398