There is a small leak above the white cartridge next to the pump. Millipore does not sell a replacement cartridge, they want us to buy a whole new pump assembly! Does anyone know what kind of filter it is and where to find a replacement?
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...
08 July 2024 1,900 1 View
I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...
24 June 2024 6,079 4 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
Hi, In a previous question I asked how to effectively model springs in Abaqus. However, I figured out the feature under Assembly -> springs/dashspots -> node to node Springs. I have a cylinder...
20 June 2024 892 3 View
Hi, I am trying to complete a buckling analysis on a tube with an external pressure. This solves itself easily by adding radial constraint on the internal edge of the tube, one axial constraint...
17 June 2024 8,165 7 View
I'm interested in the earliest days of placental terrestrial locomotion in large herbivorous taxa. Phenacodus seems to be transitional in this regard. Any others?
06 June 2024 5,182 2 View
Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...
21 May 2024 9,035 0 View
Sometimes TCSPC curves are analyzed using the actual photon counts, and sometimes they are normalized with the signal running from 0 to 1, or even from 0 to some value less than 1. What quantity...
20 May 2024 4,277 0 View
I am currently using CASTEP version 22.11, and I encounter the following error when running calculations with spin-orbit coupling: Error with dsytrf in nlpot_prepare_precon_ks Current trace...
19 May 2024 4,236 5 View
SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...
01 August 2024 5,562 2 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression,Tarim Basin of Xinjiang,NW China. Palaeontologia Sinica, New Series A,no. 14(Whole no. 190)....
28 July 2024 6,390 1 View
Hi, I am currently a upcoming 4th year student who is need of your help as I couldn't find any accessible file for the manual scoring and interpretation for the PBOI - White Campbell. My group and...
23 July 2024 2,269 1 View
isolation of microplastic from sludge sample using centrifugation ..
23 July 2024 6,418 0 View
I ask Chinese seismologists to inform me: who should I contact to create a joint Forecast Center? I do not hide that the beginning of my work was connected with the successful forecast of the...
22 July 2024 2,276 1 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
After World War, we have seen the formation of the United Nations. The sovereign body for peacekeeping but with regards to the Russia-Ukraine war it is visible that the UN seems to be ineffective...
18 July 2024 4,674 4 View