Suitable human neuronal cell line for neurotoxicity assay?

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How to evaluate the crystallinity density and amorphous density of a polymer blends by using dsc thermogram ?

Xc= (&-&a)/(&c-&a) and we have Xc 32% and &=0.91g/cc calculate the &a (amourphous density )= ? &c (crystalline density of polymer)= ?

19 January 2023 2,973 3 View

Is there anybody who has STATA or R do file to calculate Buy and Hold Abnormal Return (BHAR) and Calendar Time Portfolio Return(CTPR)?

Both BHAR and CTPR are standard methodology widely followed to measure the long run firm performance.

21 December 2022 8,822 0 View

What is an appropriate measure of multivariate function bias towards any of its input variables ?

This is in context of the objective function of a multivariate optimization problem say, f(a,b,c). I am looking for a "measure" for the degree of bias of f(a,b,c) towards any of the input variables.

15 November 2022 7,522 4 View

Can anyone help me with a company/vendor name who does whole proteomic studies (either by iTRAQ or TMT) in India?

I need to do the proteomic profiling of some of my experimental samples. Please let me know if anyone has the contact number or email ID of such service providers. Thank you!

20 October 2022 10,023 0 View

How to do FE analyse of aluminium and steel diffusion bonding?

Finite element simulation of diffusing bonding of steel and aluminium subjected to temp of 500 0c and load of 15tonne in vacuum.

03 March 2020 1,511 2 View

Does gaussian ignore imaginary frequency while calculating thermochemical properties of transition state?

Transition state from Gaussian QST3 must have imaginary frequency. For zero point energy correction if someone takes this frequency then it'll be erroneous. My concern is that whether Gaussian 16...

25 August 2019 2,464 2 View

Can anyone suggest modifications in this circuit intended for power supply ripple filtering ?

Hi, I have a design for source-follower low pass filter which I intend to use with a switched mode power supply. I simulated the circuit in NI Multisim, and for square wave ripple voltage of...

03 January 2019 3,261 9 View

How to model diffusion bonding of impeller vane by FEA ?

literature, FE modelling / analysis, failure

15 September 2018 6,865 1 View

What is Multifractal IDW interpolation method? Is this a ordinary IDW interpolated layer reclassifed by the results obtained from a fractal method?

I came across this term in a paper by A Lima 2003. Please make me understand the basic concept and how can I apply it in continuous field.

17 June 2018 1,329 0 View

Does counterpoise correction not consider electron delocalization between two fragments if there is a possibility of electron delocalization ?

I want to calculate the complexation energy of two stacked aromatic system. If I use counterpoise correction then does it not consider non covalent electron delocalization (n to sigma* or pi to pi*) ?

02 January 2018 3,600 0 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Sandip Chakraborty

You can please refer to the links below to obtain certain valuable informations:-

1. http://www.ncbi.nlm.nih.gov/pubmed/24705302

2. http://www.ncbi.nlm.nih.gov/pubmed/23917397

3. http://alttox.org/mapp/toxicity-endpoints-tests/neurotoxicity/emerging-science-policy/

Nishith Shrimali

For such studies, SH-SY5Y OR IMR-32 cell line can be used.

Satyabrata Pany

Thanks Sandip and Nishith