Suitable concentrations of apamin and paxilline for topical cortical application?

13 September 2017 1 6K Report

Hi all,

Do you guys have any experience with modulating cortical BK and SK calcium-activated potassium channels in vivo in mice? In particular I'm interested to know what would be suitable concentrations for paxilline (BK) and apamin (SK) for topical application, when aiming to manipulate neurons in L2/3?

Thanks a lot in advance.

Best

Rune

Sheng-Nan Wu

Dear Rune Rasmussen:

In vitro cell culture system, paxilline at a concentration of "1 microM" is able to block the activity of BK(Ca) channels in most of cell types.

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