Suggestions for ways of depositing microbiome sequencing data?

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Lithium ion battery Equivalent circuit parameter estimation?

I tried parameterizing an ECM model with an EIS experimental data set using Matlab lsqcurvefit solver. The resulting EIS is in an acceptable range if you see the attached figures. But my doubt...

27 March 2024 4,292 0 View

EIS behaviour for different charge rates for Lithium ion battery DFN model ?

Hello Everyone, I am obtaining the EIS from the DFN model and the EIS profile is shifting to the right side when we increase the charge C rate from 0.3 to 1C or 3C (refer to the figure below)....

05 January 2024 8,808 2 View

What do you think about hyper-aesthetics?

What is the nature of hyperaesthetics? What themes and issues do they address? What theme is cosmetic surgery there? What object is a woman? What achievements have they been given? What problems...

28 December 2023 5,176 0 View

Applying Design Science Research methodolagy in Social science applications?

Extensive research and literature indicate that CRM deployments are at an all-time high. However, there is a disparity between expectations and actual outcomes. The field of IS is paying...

28 September 2023 1,584 0 View

Do the dairy farmers’ competencies(attitudes, knowledge and skills) affect on improvement of milk production?

In this study I wanted to find out what factors that can be change milk yield of dairy farmers and do farmer’s attitudes, skills on farming, knowledge on farming relate with yield of milk...

09 August 2023 5,811 5 View

Fibre supplementation in clinical trials?

We are designing a clinical trial with fibre supplementation for humans. Is there anyone with expertise in that area to be involved as a collaborator? Someone with a nutrition background and...

23 February 2023 9,555 5 View

Does any expert have an idea about steam driven domestic cooker to replace LPG cookers and Biomass fired stoves?

I am proposing to introduce steam driven cookstoves instead of LPG cookers to overcome the community suffering current issue of LPG in my country. It is planning to operate a cluster of stoves in...

27 September 2022 6,484 6 View

How much 0.2% Saponin should I add to lyse THP-1 derived macrophages per well in a 96 well TC plate?

How much 0.2% Saponin should I add to lyse 1X 105 THP-1 derived macrophages per well in a 96 well TC plate? Most of the papers referred to do not mention a volume or ratio. Only one paper...

27 August 2022 626 0 View

How to prepare 0.2% Saponin for lysis of THP-1 derived macrophages?

I have planned to infect THP-1-derived macrophages with Mycobacterium tuberculosis. After infection, cells would be lysed to quantify intracellular viable bacteria. How should I prepare 0.2%...

25 August 2022 3,809 3 View

Mobile technology - Does Drone technology can help in transporting pathology samples from rural and remote areas??

Dear researchers, I am interested in the potential applications/benefits/barriers of implementing drone technology for transporting pathology samples from a hospital, clinic or a hub to a close-by...

07 June 2022 7,640 9 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

How I can add anew research in my account ?

Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?

21 July 2024 7,545 0 View

What is the difference between OTU and ASV analysis?

For microbiome data analysis

13 July 2024 4,542 2 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

How to prepare materials before protein-peptide or protein-protein docking?

Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...

10 July 2024 8,037 1 View

How do the complex dynamics of the oral microbiome interact with the host immune system affect periodontal disease development and treatment response?

This research question explores the complex dynamics of interactions between the oral microbiome and the host immune system, particularly in the development and treatment response of periodontal...

09 July 2024 7,922 1 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

How can I find Qiime2 bibliographic sources of updated commands?

I'm making my research on metagenomics and I want to find the source of the command 'vsearch merge-pairs' that used to be 'vsearch join-pairs'' How can I cite that?

28 June 2024 3,960 1 View

Hello, currently I am working to develop biopesticide product use Metarhizium. I would like to know how to formulate it so it can be effective?

Currently, I am working in oil palm plantation industry. This Metarhizium will be used to control the population of pest Oryctes rhinoceros. I need clue to formulate Metarhizium so it can be...

24 June 2024 9,926 3 View

Sandeep RAJ R

PLz refer our paper in extremophiles, it can be downloaded from my account.. softwares are there

Alison Dicker

I am currently submitting to the NCBI Sequence Read Archive (http://trace.ncbi.nlm.nih.gov/sra) but have also been recommended to use MG-RAST (http://metagenomics.anl.gov/).

Thilini Jayasinghe

Sandeep RAJ R and Alison Dicker, Thanks a lot for taking time to answer my question.

Thilini

Claudia Wultsch

NCBI Sequence Read Archive (http://trace.ncbi.nlm.nih.gov/sra) would work and in case you use Ilumina basespace..it offers an SRA submission app

Thank you, Claudia.