House-keepers might not be good reference genes, and a good reference gene in one study may be a bad one in another study.

If a gene is a good reference gene depends on the experimental conditions you want to study. It must be expressed in the cell type you are studying and it must be constantly expressed in all experimental conditions.

So if you ask for reference genes that would be ok for your experiment, you must specify the different experimental conditions you want to compare.

Good reference genes are hard to identify, and the usual recommendation is to use a whole panel of different (hopefully, presumably) good reference genes.

I agree with you Jochen Wilhelm

Thanks!

Hi Heleen! For T cells and neutrophils I found these 2 papers:

https://www.ncbi.nlm.nih.gov/pubmed/15182252

https://www.ncbi.nlm.nih.gov/pubmed/22011438

Best!

In the end, we selected three housekeeping genes (B2M, SDHA, and TBP), carefully chosen from a list of 8 reference targets, also including ACTB, IPO8, PGK1, RPL13, and RPS14, using geNorm in qBase+ (geNorm V < 0.15; Version 2.6.1, Biogazelle, Gent, Belgium). Ref: Van Acket et al. Cancers 2019, 11(7), 1029; https://doi.org/10.3390/cancers11071029

I recommend "Housekeeping and Reference Transcript Atlas"(www.housekeeping.unicamp.br) for PBMC. It is a web-based database of human and mouse housekeeping genes which offers tissue/cell (102 human tissues and 22 mouse tissues at this moment) selective candidate reference transcripts for qPCR normalization and some validated ready to use primers.