Ivan Kadurin

11 Questions 7 Answers 0 Followers

Questions related from Ivan Kadurin

Anyone advise IRES vs P2A sites or separate promoters for bi-cystronic AAV vector?

I am about to order a vector for bi-cystronic expression of peptide of interest and GFP as a marker. I will only use GFP as a marker to select infected cells. Does anyone gave experience with IRES...

18 December 2024 2,134 0 View

Can someone suggest statistical methods to exclude outliers?

I am looking for tested methods to exclude outliers statistically, if someone could help with suggestions of methods. Links to instructions / clarifications will be appreciated Thanks in advance

25 August 2023 2,146 4 View

Suggestions for epitope tags/Antibodies for Immunocytochemistry (HA, VSV, c-Myc etc)?

I would like to add an epitope tag to my protein (internal, not C- or N-terminal) for imunocytochemical labelling. Do you have suggestions for specific epitopes / antibodies that may work well,...

31 May 2023 9,980 0 View

Variable pipette size / how to fix unstable program Sutter Pipette Puller P-97?

We recently replaced the heating filament of our P-97 puller. Since then the programs have been producing variable pipette ends with same program. Every time the puller switches off we have to...

30 January 2023 4,553 2 View

Which power supply should I buy for a halogen bulb (electrophysiology rig) ?

I have to equip my microscope with a power supply for a halogen bulb illuminating my samples from above. Can you suggest something ?

27 July 2022 613 4 View

Can you recommend site for used lab equipment in UK?

Please suggest websites other than ebay to buy used lab equipment. Especially interested in novex invitrogen powersupply or electrophysiology equipment. Thanks

28 April 2022 7,209 1 View

Can anyone help with converting file of protein structure for 3D printing ?

I have the file in Pymol for example or as a PDB image . Can someone provide a guideline how this can be converted for 3D printing ? Thanks in advance

05 May 2021 5,464 8 View

Anyone know the concentrations of Thrombin in blood plasma ?

Can somebody tell me (or send me a link to the literature) what are the normal concentrations of active Thrombin in blood (in enzyme Units) ? Also, what are the physiological...

18 November 2015 810 10 View

Does anyone have experience with photocaged unnatural amino acids?

I would like to express a protein in HEK cells with photocaged unnatural cysteine encoded. I am interested in expression vectors and protocols. Do you have any recommendations for papers to read,...

20 April 2015 9,762 0 View

Does anyone have experience with Rotenone in a biochemistry lab (micromolar concentration)?

Some metabolic experiments require Rotenone, but I would like to get some information about solubility, toxicity and lab handling of this compound before planning to do anything with it. Thanks

19 June 2014 3,478 0 View

I would like to transfect HEK cells with siRNA. Can I use Fugene6 or PolyJet or do I need specific transfection reagent ?

08 June 2013 722 2 View