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Does anyone have experience with Mouse Anti BrdU Immuno protocol? I am working on young rat's brains exposed to prenatal BrdU (i.p.). Literature available are confusing. Will I be required to...
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The tissue were perfusion-fixed with 4% PFA after PBS washing and the rat brains were remove and post-fixed for 48 hours. Will it be necessary or not to allow antigen retrieval before proceeding...
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Hello, I intend to prepare rat's fetal and pup brains for immunohistochemistry and immuofluorescence. I am required to inject the dams with BrDu, but I don't know how to prepare the solution. Can...
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