I have used mild as well as harsh stripping buffer. Also, 5mM EDTA stripping for western blot. Anyone here who can suggest, best buffer for primar antibody removal from blot along with protocol.
Thanks in advance.
Colony formation capacity of cells suing siRNA. As cells are incubated for 7 to 14 days, by adding FBS it reduces or revert transfection. Please find attached my optimized protocol.
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Transfection using siRNA for cell lines.
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