Hello, I want to induce DT2 in rats. I read a lot of articles on this subject and I understood that the dosage of STZ and the time between STZ and NA administration is very important; In almost all articles that I read I found that the proper dose of NA is between 90-140 mg/kg. Masiello P. (1998) who developed this model has used 230 mg/kg NA. What is the reason for such a large difference in dosages between the two? PS: STZ dosage is similar in all cases 40-65 mg/kg" Thank you very much
Our research team has dealt with this protocol for years. Pilot experiments are needed in order to find the proper combination dosage of STZ and NA, since different results are obtained from lab to lab. Don't be so sure that the achieved diabetes will be the type 2. Also,mind that STZ is photo(light)-sensitive and must be administered freshly after its preparation. Very long fasting of the animals doesn't help. Not all the animals will be rendered diabetic. In my opinion models combining high-fat diet and (a) low dose(s) of STZ are more appropriate for the study of type 2 diabetes. To my mind, the model you are asking about will easily turn the animals into type 1 diabetes.
Please, get acquainted with the views of the scientists on the subject (manuscripts in the annex)
Nicotinamide has been shown to be of proven effectiveness across a wide range of doses, even in humans.
I have induced diabetes in rats using STZ alone, that method has its challenges. Please, I will be interested in the outcome of the your method. All the best.
Our research team has dealt with this protocol for years. Pilot experiments are needed in order to find the proper combination dosage of STZ and NA, since different results are obtained from lab to lab. Don't be so sure that the achieved diabetes will be the type 2. Also,mind that STZ is photo(light)-sensitive and must be administered freshly after its preparation. Very long fasting of the animals doesn't help. Not all the animals will be rendered diabetic. In my opinion models combining high-fat diet and (a) low dose(s) of STZ are more appropriate for the study of type 2 diabetes. To my mind, the model you are asking about will easily turn the animals into type 1 diabetes.
Numerous studies have also demonstrated that the STZ-induced increase in blood glucose is significantly blunted when NA is administered prior to STZ. The advantageous effect of NA on blood glucose is due to the protection of B-cells against demage of STZ and is accompanied by increased blood insulin.
The severity of diabetes in experimental rats strongly depends on the doses of STZ and NA given to these animals.
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