Logaritmic phase (exponential growth phase)...800ul of your colture plus 200ul glicerol 100% = 1 ml total (glicerol 20%) in screw cap tubes for -80°C....chill in ice before put'em in -80°C.
I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...
04 November 2020 9,038 2 View
I have a VCF file with SNPs using the IGV program, when uploading the file some SNPs that are observed in a particular gene in IGV, when verifying it in GDV or Ensembl do not match
10 August 2020 699 3 View
Dear colleagues, I am currently working on a health's blockchain able of integrating informations relating to COVID-19 patients. The difficulty is to allow this blockchain to be a general...
21 April 2020 8,492 4 View
I want to do citation analysis on papers on ecnomics, but I don't know exactly how to get this data from Web of Science, though many sources say this is one of the very good datasets in this area....
16 March 2020 4,184 4 View
I would like to track the presence of some ideas in papers in economics, and I have been reading about citation network analysis and I have found some datasets (like the...
26 February 2020 2,848 1 View
A NGS panel of truseq was performed, the SNP expected of CSN1S2, Ch 6, Position 87266177 are C/T, but we received A/G. There is an explain about that?
28 January 2020 1,118 2 View
Dear RG community, I am looking for anti-rat FC blocking antibodies (CD16/C32) for the QC of a FACS immunophenotyping experiment. So far, I can only find anti-mouse Fc block from manufacturers...
04 December 2019 7,336 1 View
Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?
06 November 2017 7,938 2 View
11 February 2016 4,772 1 View
We are performed an electrophoresis with primers taken from a paper based on hemiparasites samples. The posterior electrophoresis was different from the original work 160bp vs 700bp in several...
27 October 2015 4,544 6 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...
20 February 2021 6,897 1 View
I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...
15 February 2021 7,444 2 View
I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...
14 February 2021 7,800 7 View
I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...
09 February 2021 8,594 3 View
In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...
08 February 2021 2,792 1 View
Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...
07 February 2021 9,530 3 View
I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...
04 February 2021 1,509 3 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View