I want to know how to calculate statistical differences between values and represent results with letters (a, b, c,....)
Try SAS analysis. No hindrance at all, you will get what you want.....
This is 'Duncan grouping' in SAS
https://support.sas.com/documentation/cdl/en/statug/63347/HTML/default/viewer.htm#statug_anova_sect027.htm
In attachment the SAS program. Nowadays SAS uses coloured bars.
SAS can be run online:
https://www.sas.com/en_us/software/on-demand-for-academics.html
I recommend that you can use SPSS to calculate the differences, like T-test, variance analysis, kappa-test.
Thank you very much for your help
You Can also use LSD test in Statistica or minitab software
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