Hi! I'm isolating cardiomyocytes from adult mice and need to do live cell staining. I'd like to use myomesin as my cardiomyocyte marker (antibody: https://dshb.biology.uiowa.edu/mMaC-myomesin-B4). Considering that the cells won't be permeabilized, has anyone tried staining for myomesin or other cardiomyocyte marker in live cells? Any advice would be greatly appreciated. Thank you.

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