Out of date Agilent RNA 6000 kit?

I have a colleague who wants to run QC on a few RNA samples. We have an unused RNA 6000 kit. Unfortunately The chips and the associated reagents all went out of date 3 months ago. Everything...

05 June 2019 2,262 3 View

Staining FFPE sections with anti human STING (TMEM173) antibody?

I have identified a couple of clones from Abcam: Rabbit monoclonal clone SP338, Rabbit monoclonal clone SP339: and two Human Protein Atlas approved clones available via Sigma: Rabbit polyclonal...

02 March 2019 3,331 2 View

Anti-human CLEC4F?

Can anyone recommend an alternative to the anti-human CLEC4F antibody currently supplied by Abcam, ctalogue number ab170184? Also, can anyone recommend an optimal tissue to titrate the antibody...

08 September 2018 3,940 2 View

Can anyone suggest an alternative to Liberase TL?

I currently use Liberase TL to digest collagen droplets, and also isolate TILs from tissues. This product is currently not available from Sigma (the sole supplier), Liberase TM is too harsh, and...

31 December 2016 215 5 View

Shelf life of Leica CV ultra mounting media?

I am using Leica CV ultra to mount cover-slips. I noticed that it is 3 months past its shelf life (expiry date 9/2016). Is it OK to continue using it?

11 December 2016 1,556 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

No title

01 March 2021 3,905 5 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View