Out of date Agilent RNA 6000 kit?

I have a colleague who wants to run QC on a few RNA samples. We have an unused RNA 6000 kit. Unfortunately The chips and the associated reagents all went out of date 3 months ago. Everything...

05 June 2019 2,262 3 View

Staining FFPE sections with anti human STING (TMEM173) antibody?

I have identified a couple of clones from Abcam: Rabbit monoclonal clone SP338, Rabbit monoclonal clone SP339: and two Human Protein Atlas approved clones available via Sigma: Rabbit polyclonal...

02 March 2019 3,331 2 View

Anti-human CLEC4F?

Can anyone recommend an alternative to the anti-human CLEC4F antibody currently supplied by Abcam, ctalogue number ab170184? Also, can anyone recommend an optimal tissue to titrate the antibody...

08 September 2018 3,940 2 View

Staining FFPE sections with anti CAIX antibody (clone M75)?

Has anyone got experience of staining FFPE sections with anti CAIX antibody (clone M75)? Specifically information regarding the dilution of antibody to use, and the preferred antigen retreival...

06 July 2018 3,971 3 View

Can anyone suggest an alternative to Liberase TL?

I currently use Liberase TL to digest collagen droplets, and also isolate TILs from tissues. This product is currently not available from Sigma (the sole supplier), Liberase TM is too harsh, and...

31 December 2016 215 5 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

No title

22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

A film coverage that allows for transmittance of wavelengths on the infrared scale?

I've landed on needing some type of highly optically transparent film or cover material that prevents evaporation of culture media; I am not sure which subfield of materials science would be able...

08 February 2021 5,760 1 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

How to create a Hypothesis and Thesis in a statement?

03 February 2021 4,496 3 View