When ssr primers as a QTL flanking markers find the polymorphism between parents that shows the difference of almost 200 bp is more good for fine mapping or a little difference 20-30 bp between parents is also consider to be good? please explain
A photo is attached please see as a reference ..
Regards
Dear Dr. M Razzaq,
Greetings, I would like to thank you for your question. Better find polymorphism and low differences means the same allele in the same chromosome. Some times SSR markers have different alleles in different chromosomes.
Also, few genes/QTLs have cloned using fine mapping
with best regards
Khaled
@Khaled... So it's mean 200 BP or almost 10-30 BP Polymorphism not matters? Right?
Regards
Dear Muhammad Khuram Razzaq,
10-30 bp polymorphism is better than 200 bp, and that difference is more probably for alleles from the same chromosome. If the difference between alleles is grater then it is probably those alleles belong to different loci.
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